AMPK调控绵羊肌内前体脂肪细胞分化的研究

doi:10.11843/j.issn.0366-6964.2018.08.004

Abstract

Abstract:

The objective of this study was to investigate the roles and mechanisms of AMP-activated protein kinase(AMPK) in sheep muscle derived preadipocytes differentiation. The longissimus dorsi(LD) muscle was sampled from lamb of 3 days old, and the perimysium was isolated and digested for preadipocytes isolation by differential adhesion method. The activity of AMPK and Wnt/β-catenin signaling pathways were altered, and then the cells adipogenic differentiation was induced. qRT-PCR and Western blot were used to detect the expression patterns of key genes and proteins in preadipocytes differentiation. Oil-Red-O staining was used to observe the lipid droplet accumulation in mature adipocytes. The results showed that activating AMPK was associated with less Oil-Red-O staining. Meanwhile, the expressions of adipogenic genes and corresponding proteins including PPARγ, C/EBPα and aP2 were down-regulated at significant level of 0.01 when AMPK was activated, while inhibition of AMPK activity showed opposite effects. The activation of Wnt/β-catenin signaling pathway inhibited preadipocytes adipogenesis, decreased the expressions of PPARγ and C/EBPα mRNA at significant level of 0.01, and down-regulated aP2 gene expression at significant level of 0.05, moreover, the corresponding protein contents were also decreased at significant level of 0.01. Furthermore, inhibition of Wnt/β-catenin signaling pathway promoted adipogenesis. β-catenin transcription was not affected by alteration of AMPK activity but β-catenin protein content significantly altered(P<0.01). Finally, AMPK activation increased GSK3β phosphorylation at significant level of 0.01. The result indicated that AMPK regulated sheep muscle derived preadipocytes differentiation through altering GSK3β phosphorylation and affecting the stability of β-catenin.

CLC Number:

S826.2

LI Kan, LIU Wen-zhong, ZHANG Rui-xin, LI Qian, ZHANG Ting, QIN Xu-ze, ZHANG Jian-xin, ZHAO Jun-xing. AMPK Regulates Sheep Muscle Derived Preadipocytes Differentiation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1594-1604.

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AMPK Regulates Sheep Muscle Derived Preadipocytes Differentiation

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